| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
RAPID COMMUNICATION |
a BresaGen, Inc., Athens, Georgia, USA;
b Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA;
c Department of Animal and Dairy Sciences, University of Georgia, Athens, Georgia, USA;
d Reproductive Biology Associates, Atlanta, Georgia, USA;
e Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, USA;
f Department of Genetics, University of Georgia, Athens, Georgia, USA
Key Words. Embryonic stem cell • Differentiation • Discarded embryo
Maisam Mitalipova, Ph.D., BresaGen, Inc., 111 Riverbend Road, Georgia Biobusiness Center, Athens, Georgia 30605, USA. Telephone: 706-613-9878, ext. 279; Fax: 706-613-9879; e-mail: mitalipova{at}hotmail.com
Human pluripotent embryonic stem (ES) cells have important potential in regenerative medicine and as models for human preimplantation development; however, debate continues over whether embryos should be destroyed to produce human ES cells. We have derived four ES cell lines on mouse embryonic fibroblast cells in medium supplemented with basic fibroblast growth factor, human recombinant leukemia inhibitory factor, and fetal bovine serum. The source of these cell lines was poor-quality embryos that in the course of routine clinical practice would have been discarded. After continuous proliferation in vitro for more than 12 months, these ES cell lines maintained their developmental potential to form trophoblast and somatic cells, including cardiac muscle and neuronal tissue.
This article has been cited by other articles:
|
|
 |
|
  S. Ziebe, P. Devroey, and on behalf of the State of the ART 2007 Workshop Gr Assisted reproductive technologies are an integrated part of national strategies addressing demographic and reproductive challenges Hum. Reprod. Update, November 1, 2008; 14(6): 583 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. D. West, D. W. Machacek, N. L. Boyd, K. Pandiyan, K. R. Robbins, and S. L. Stice Enrichment and Differentiation of Human Germ-Like Cells Mediated by Feeder Cells and Basic Fibroblast Growth Factor Signaling Stem Cells, November 1, 2008; 26(11): 2768 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Sun, X. Long, Y. Yin, Y. Jiang, X. Chen, W. Liu, W. Zhang, H. Du, S. Li, Y. Zheng, et al. Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes Hum. Reprod., October 1, 2008; 23(10): 2185 - 2193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Scott and R. A. Reijo Pera The road to pluripotence: the research response to the embryonic stem cell debate Hum. Mol. Genet., April 15, 2008; 17(R1): R3 - R9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-P. Kim, A. Thurston, C. Mummery, D. Ward-van Oostwaard, H. Priddle, C. Allegrucci, C. Denning, and L. Young Gene-specific vulnerability to imprinting variability in human embryonic stem cell lines Genome Res., December 1, 2007; 17(12): 1731 - 1742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Garcia-Perez, M. C.N. Marchetto, A. R. Muotri, N. G. Coufal, F. H. Gage, K. S. O'Shea, and J. V. Moran LINE-1 retrotransposition in human embryonic stem cells Hum. Mol. Genet., July 1, 2007; 16(13): 1569 - 1577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Boyd, S. K. Dhara, R. Rekaya, E. A. Godbey, K. Hasneen, R. R. Rao, F. D. West III, B. A. Gerwe, and S. L. Stice BMP4 Promotes Formation of Primitive Vascular Networks in Human Embryonic Stem Cell-Derived Embryoid Bodies Experimental Biology and Medicine, June 1, 2007; 232(6): 833 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Allegrucci, Y.-Z. Wu, A. Thurston, C. N. Denning, H. Priddle, C. L. Mummery, D. Ward-van Oostwaard, P. W. Andrews, M. Stojkovic, N. Smith, et al. Restriction landmark genome scanning identifies culture-induced DNA methylation instability in the human embryonic stem cell epigenome Hum. Mol. Genet., May 15, 2007; 16(10): 1253 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shin, Y. Sun, Y. Liu, H. Khaner, S. Svant, J. Cai, Q. X. Xu, B. P. Davidson, S. L. Stice, A. K. Smith, et al. Whole Genome Analysis of Human Neural Stem Cells Derived from Embryonic Stem Cells and Stem and Progenitor Cells Isolated from Fetal Tissue Stem Cells, May 1, 2007; 25(5): 1298 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Burridge, D. Anderson, H. Priddle, M. D. Barbadillo Munoz, S. Chamberlain, C. Allegrucci, L. E. Young, and C. Denning Improved Human Embryonic Stem Cell Embryoid Body Homogeneity and Cardiomyocyte Differentiation from a Novel V-96 Plate Aggregation System Highlights Interline Variability Stem Cells, April 1, 2007; 25(4): 929 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Allegrucci and L.E. Young Differences between human embryonic stem cell lines Hum. Reprod. Update, March 1, 2007; 13(2): 103 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wang, X. Tang, Y. Niu, H. Chen, B. Li, T. Li, X. Zhang, Z. Hu, Q. Zhou, and W. Ji Generation and Characterization of Rabbit Embryonic Stem Cells Stem Cells, February 1, 2007; 25(2): 481 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Brimble, E. S. Sherrer, E. W. Uhl, E. Wang, S. Kelly, A. H. Merrill Jr., A. J. Robins, and T. C. Schulz The Cell Surface Glycosphingolipids SSEA-3 and SSEA-4 Are Not Essential for Human ESC Pluripotency Stem Cells, January 1, 2007; 25(1): 54 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, P. Stojkovic, S. Przyborski, M. Cooke, L. Armstrong, M. Lako, and M. Stojkovic Derivation of Human Embryonic Stem Cells from Developing and Arrested Embryos Stem Cells, December 1, 2006; 24(12): 2669 - 2676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A De Sousa, G. Galea, and M. Turner The road to providing human embryo stem cells for therapeutic use: the UK experience. Reproduction, November 1, 2006; 132(5): 681 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Skottman and O. Hovatta Culture conditions for human embryonic stem cells. Reproduction, November 1, 2006; 132(5): 691 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mitalipov, H.-C. Kuo, J. Byrne, L. Clepper, L. Meisner, J. Johnson, R. Zeier, and D. Wolf Isolation and Characterization of Novel Rhesus Monkey Embryonic Stem Cell Lines Stem Cells, October 1, 2006; 24(10): 2177 - 2186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Huettner, A. Lu, Y. Qu, Y. Wu, M. Kim, and J. W. McDonald Gap Junctions and Connexon Hemichannels in Human Embryonic Stem Cells Stem Cells, July 1, 2006; 24(7): 1654 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Trounson The Production and Directed Differentiation of Human Embryonic Stem Cells Endocr. Rev., April 1, 2006; 27(2): 208 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cai, J. Chen, Y. Liu, T. Miura, Y. Luo, J. F. Loring, W. J. Freed, M. S. Rao, and X. Zeng Assessing Self-Renewal and Differentiation in Human Embryonic Stem Cell Lines Stem Cells, March 1, 2006; 24(3): 516 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Plaia, R. Josephson, Y. Liu, X. Zeng, C. Ording, A. Toumadje, S. N. Brimble, E. S. Sherrer, E. W. Uhl, W. J. Freed, et al. Characterization of a New NIH-Registered Variant Human Embryonic Stem Cell Line, BG01V: A Tool for Human Embryonic Stem Cell Research Stem Cells, March 1, 2006; 24(3): 531 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shin, M. Mitalipova, S. Noggle, D. Tibbitts, A. Venable, R. Rao, and S. L. Stice Long-Term Proliferation of Human Embryonic Stem Cell-Derived Neuroepithelial Cells Using Defined Adherent Culture Conditions Stem Cells, January 1, 2006; 24(1): 125 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Dvorak, D. Dvorakova, S. Koskova, M. Vodinska, M. Najvirtova, D. Krekac, and A. Hampl Expression and Potential Role of Fibroblast Growth Factor 2 and Its Receptors in Human Embryonic Stem Cells Stem Cells, September 1, 2005; 23(8): 1200 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sasaki, K. Hanazawa, R. Kurita, A. Akatsuka, T. Yoshizaki, H. Ishii, Y. Tanioka, Y. Ohnishi, H. Suemizu, A. Sugawara, et al. Establishment of Novel Embryonic Stem Cell Lines Derived from the Common Marmoset (Callithrix jacchus) Stem Cells, September 1, 2005; 23(9): 1304 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, K. Qian, J. Hu, D. Liu, W. Lu, Y. Yang, D. Wang, H. Yan, S. Zhang, and G. Zhu The derivation of two additional human embryonic stem cell lines from day 3 embryos with low morphological scores Hum. Reprod., August 1, 2005; 20(8): 2201 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Stojkovic, M. Lako, S. Przyborski, R. Stewart, L. Armstrong, J. Evans, X. Zhang, and M. Stojkovic Human-Serum Matrix Supports Undifferentiated Growth of Human Embryonic Stem Cells Stem Cells, August 1, 2005; 23(7): 895 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Stojkovic, M. Lako, R. Stewart, S. Przyborski, L. Armstrong, J. Evans, A. Murdoch, T. Strachan, and M. Stojkovic An Autogeneic Feeder Cell System That Efficiently Supports Growth of Undifferentiated Human Embryonic Stem Cells Stem Cells, March 1, 2005; 23(3): 306 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Schulz, S. A. Noggle, G. M. Palmarini, D. A. Weiler, I. G. Lyons, K. A. Pensa, A. C.B. Meedeniya, B. P. Davidson, N. A. Lambert, and B. G. Condie Differentiation of Human Embryonic Stem Cells to Dopaminergic Neurons in Serum-Free Suspension Culture Stem Cells, December 1, 2004; 22(7): 1218 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Rao and S. L. Stice Gene Expression Profiling of Embryonic Stem Cells Leads to Greater Understanding of Pluripotency and Early Developmental Events Biol Reprod, December 1, 2004; 71(6): 1772 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Pera and A. O. Trounson Human embryonic stem cells: prospects for development Development, November 15, 2004; 131(22): 5515 - 5525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stojkovic, M. Lako, T. Strachan, and A. Murdoch Derivation, growth and applications of human embryonic stem cells Reproduction, September 1, 2004; 128(3): 259 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stojkovic, M. Lako, P. Stojkovic, R. Stewart, S. Przyborski, L. Armstrong, J. Evans, M. Herbert, L. Hyslop, S. Ahmad, et al. Derivation of Human Embryonic Stem Cells from Day-8 Blastocysts Recovered after Three-Step In Vitro Culture Stem Cells, September 1, 2004; 22(5): 790 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Dobson, R. Raja, M. J. Abeyta, T. Taylor, S. Shen, C. Haqq, and R. A. R. Pera The unique transcriptome through day 3 of human preimplantation development Hum. Mol. Genet., July 15, 2004; 13(14): 1461 - 1470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zeng, T. Miura, Y. Luo, B. Bhattacharya, B. Condie, J. Chen, I. Ginis, I. Lyons, J. Mejido, R. K. Puri, et al. Properties of Pluripotent Human Embryonic Stem Cells BG01 and BG02 Stem Cells, May 1, 2004; 22(3): 292 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Heins, M. C.O. Englund, C. Sjoblom, U. Dahl, A. Tonning, C. Bergh, A. Lindahl, C. Hanson, and H. Semb Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells Stem Cells, May 1, 2004; 22(3): 367 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pennings and A. Van Steirteghem The subsidiarity principle in the context of embryonic stem cell research Hum. Reprod., May 1, 2004; 19(5): 1060 - 1064. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
