Cover Archive 

Volume 28 Issue 1

iPS cells generated using a constitutive single excisable lentiviral stem cell cassette (STEMCCA-loxP) show normal colony morphology and robust alkaline phosphatase activity. See Sommer et al. beginning on page 64.

Volume 27 Issue 12

Human ES cell-derived neural differentiation culture. CD29 surface antigen (green) is highly expressed on Pax6-positive neuroepithelial cells (red), and also strongly present on a population of mesenchyme/neural crest-like proliferative cells (doublecortin-negative). In contrast, differentiating neuroblasts (doublecortin; blue) exhibit negative/ low CD29 expression. Combinatorial flow cytometric analysis of CD29 in conjunction with CD15 and CD24 surface markers enables separation of these three distinct subsets by FACS. See Pruszak et al. beginning on page 2928.

Volume 27 Issue 11

Numb overexpression does not down regulate Notch signaling during somitogenesis. Whole-mount in situ hybridization was done with an antisense probe for HeyL on mouse E11.5 caudal somites in the tail. Note that overexpression of Numb does not alter the expression of this Notch target gene in the transgenic (Tg:6X::3XEpE-NbGFP(IHR)) embryo. Immunostaining with anti-GFP of the tail of E10.75 transgenic embryo (inset) shows high expression of NumbGFP in presomitic mesoderm and somites. See Jory et al. beginning on page 2769.

Volume 27 Issue 10

Fresh satellite cells stripped from single fibers of young mice efficiently regenerate and self-renew when injected in irradiated mature adult mdx-nude muscles. Four hundred satellite cells physically dissociated from extensor digitorum longus myofibers of 3F-/nLacZ/-2E and Myf5nLacZ/+ mice were injected into the irradiated tibialis anterior(TA) ofmdx-nude recipient mice. These gave rise to regenerated muscle fibres, expressing dystrophin. See Boldrin et al. beginning on page 2478.

Volume 27 Issue 9

Dissociated rat olfactory mucosa plated in neural stem cell media form two types of spheres. Immunofluoresence image of olfactory mucosa-II sphere labeled with anti- cytokeratin (green), connected to olfactory mucosa-I sphere labeled with anti-fibronectin (red), and DAPI (blue). See Tome ́ et al. beginning on page 2196.

Volume 27 Issue 8

Examples of normal and aberrant mitotic figures visualized by confocal microscopy following c-irradiation of human embryonic stem cells. Normal mitotic spindle structures are obderved including metaphase, anaphase and telophase. Addtionally aberrant structures are observed including misaligned chromosomes, multipolar spindles and anaphase bridges (not shown). Green, histone H3-serine 10; red, b-tubulin. See Momcˇilovic´ et al. beginning on page 1822.

Volume 27 Issue 7

Rescue of intravascular hematopoietic cell clusters in the umbilical cord of E11.5 [Tie2-Cre: Runx1/LacZ/LacZ] embryos. See Liakhovitskaia et al. beginning on page 1616.

Volume 27 Issue 6

Immunohistochemical staining of the developing mouse eye shows that proliferating (PCNA+) cells are restricted to the outer nuclear layer and the ciliary marginal zone (CMZ), while phosphoCREB is expressed in the inner nuclear layer and the CMZ. See Dworkin et al. beginning on page 1347.

Volume 27 Issue 5

CD44 immunohistochemistry of hypergastrinemic INS-GAS mouse stomach with Helicobacter felis infection (top or left) and human gastric adenocarcinoma of moderately differentiated type (bottom or right) shows that the invasive front area of both mouse and human gastric cancer tissues are strongly CD44 positive. See Takaishi et al. beginning on page 1006.

Volume 27 Issue 4

The periventricular region of the 3rd ventricle of adult mice brain contains no structured subventricular region, but densely packed nuclei of ependymal type E cells (blue), astrocytic type B neuroprogenitors with extensions projecting towards the hypothalamus (GFAP in green), and occasional SSEA1+ cells outside the ventricular lining (red). See Hermann et al. beginning on page 928.

Volume 27 Issue 3

Stem cell and differentiation markers in murine hair follicle and cornea; expression of K15, a putative epithelial stem and progenitor cell marker, in the hair follicle bulge and basal cells. Nuclear staining performed with propidium iodide (red) or 4,6-diamidino-2-phenylindole (blue). See Blazejewska et al. beginning on page 642.

Volume 27 Issue 2

Shown are sections of 2 days old human embryoid bodies as bright field (right) or after immunostaining (left) for LIN-28 expression (red staining), OCT4 expression (green staining) and DAPI staining for DNA content (blue). See Darr and Benvenisty beginning on page 352.

Volume 27 Issue 1

HESR dish culture preserves human mesenchymal stem cell (hMSC) potential to differentiate into mesenchymal cell lineages. Myogenesis was detected by immunofluorescence staining for smooth muscle myosin heavy chain (green) and α-smooth muscle actin (red) in a striated stress fiber pattern. See Majd et al. beginning on page 200.

Volume 26 Issue 12

Sample mammospheres formed after 10 days in culture from early parous or age-matched virgin mice contained keratin 8+ cells; approximately one in three also contained keratin 5+ cells. See Siwko et al. beginning on page 3205.

Volume 26 Issue 11

Secreted Frizzled-related protein-1 (sFRP1) modified mesenchymal stem cell (MSC) morphology and increased ß-catenin staining in homotypic and heterotypic cell-cell junction. MSC were cocultured with endothelial cells or with smooth muscle cell (SMC) for 48 hours, with recombinant bovine (rb) sFRP1. Cells were fixed and stained for green fluorescent protein (GFP; localization of MSC, green), ß-catenin (red), and nuclei (blue). MSC interacted with both cell types through ß-catenin cell-cell junctions. sFRP1 treatment increased ß-catenin staining at cell-cell junctions in the coculture.

Volume 26 Issue 10

JK1 cells support the maintenance of murine embryonic stem cells. Immunofluorescence demonstrated that mESC-derived teratomas expressed markers of all three germ layers: NeuN (left panel); GFAP (right panel). Antibody staining is green and nuclear counterstain is blue. See Kim et al. beginning on page 2516.

Volume 26 Issue 9

A staining of hESC-NL3 for OCT3/4a in red, Collagen I in green, and DNA in blue. See Braam et al. beginning on page 2257.

Volume 26 Issue 8

Differentiation of murine mobilized very small embryonic-like (VSEL) stem cells into cardiomyocytes. Granulocyte colony-stimulating factor (GFP)-positive VSEL stem cells were cocultured with GFP-negative bone marrow stromal cells in cardiac differentiation media. Expression of α-sarcomeric actinin was detected by red fluorescence. Nuclei were identified by 4,6-diamidino-2-phenylindole. See Kucia et al. beginning on page 2083.

Volume 26 Issue 7

Cardiomyogenic differentiation of human menstrual blood-derived mesenchymal cells (MMCs) was observed in vitro. Immunocytochemistry revealed a clear striation pattern of cardiac troponin-I (red) in about 30% of GFP-positive (green) MMCs. The GFP and troponin-I staining were observed alternately in a striated manner, suggesting that troponin-I is expressed in the GFP-positive cells. See Hida et al. beginning on page 1695.

Volume 26 Issue 6

Androgenetic embryonic stem (ES) cells contribute to fetal brain development following blastocyst injection. Shown is an immunostaining of a sagittal cryosection of an embryonic day 12.5 chimeric brain (green: androgenetic ES cell-derived cells; red: neurons; blue: nuclei) See Dinger et al. beginning on page 1474.

Volume 26 Issue 5

Immunophenotype of a terminal bronchiole following progenitor cell depletion. Repairing epithelium of wild-type mice (shown) includes a rare population of cells that stain for both CCSP (green) and proSPC (red). Potentiation of ß-catenin signaling leads to a dramatic expansion of these cells. See Reynolds et al. beginning on page 1337.

Volume 26 Issue 4

Interstitial stem cells infiltrating tumor necrosis factor-α-treated, regenerating skeletal muscle, exhibit active caspases (red) and rarely coexpress activated caspase-3 (green). Hoechst (blue) stained nuclei; orange stained laminin. See Moresi et al. beginning on page 997.

Volume 26 Issue 3

Human umbilical cord stroma-derived stem cells in an undifferentiated state at day 28 in culture exhibit constitutive lipid granules (intracytoplasmic red foci), fine arrays of stress fibers (green filaments), and nuclei (blue). See Karahuseyinoglu et al. beginning on page 682.

Volume 26 Issue 2

Development of human cloned blastocysts following somatic cell nuclear transfer (SCNT) with adult fibroblasts (AF). Late day 3 SCNT embryo following nuclear transfer with AF donor cell. See French et al. beginning on page 485.

Volume 26 Issue 1

Cells of the rat vibrissal follicle dermal papilla express fibronectin in vivo (center, green; nuclei counterstained with Hoechst, blue). The dermal papilla of the hair follicle is a highly enriched niche of sphere-forming neural crest precursor cells in adult skin. See Hunt et al. beginning on page 163.